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KMID : 1005220090240020115
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Journal of Embryo Transfer
2009 Volume.24 No. 2 p.115 ~ p.119
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Study on In Vitro Development of Vitrified-Thawed Porcine Oocytes
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Chung Gun-Ho
Rhee Man-Hee
Kim Sang-Keun
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Abstract
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In the present study, effects of concentration of cryoprotectant solutions on the nuclear maturation of vitrifiedthawed porcine oocytes were examined. Oocytes were cultured in TCM-199 medium supplemented with 5% FBS at C in 5% and air. The percentage of monospermy in the toxicity group and vitrification group (22.0 3.0% and 31.5 3.5%) was decreased compared with that of the control group (44.0 4.0%). The percentage of in vitro development to blastocyst in the toxicity group and vitrification group (12.0 2.5% and 14.8 2.8%) was decreased compared with that of the control group (28.0 3.0%, p<0.05). The survival and in vitro developmental rate of oocytes vitrification-thawed with EDS and EDT + TCM-199 medium supplemented with 0.1% PVA were 46.3 3.0%, 54.5 3.8% and 14.8 2.5%, 16.4 2.7%, respectively. This results were lower than the control group (28.0 3.5%). The in vitro developmental rate of embryos vitrified with EDS and EDT supplemented PVA did not have a significant difference. The survival and in vitro developmental rate of vitrified-thawed morula and blastocyst embryos were 44.2 3.5%, 17.3 3.0% and 48.1 4.2%, 18.5 3.5%, respectively. Vitrified morulae and blastcyst embryos had a lower survival and developmental rates than their control counterparts.
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KEYWORD
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porcine oocytes, vitrification, survival and in vitro development
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